Stage- and Gender-Specific Proteomic Analysis of Brugia malayi Excretory-Secretory Products
Identifieur interne : 006931 ( Main/Exploration ); précédent : 006930; suivant : 006932Stage- and Gender-Specific Proteomic Analysis of Brugia malayi Excretory-Secretory Products
Auteurs : Yovany Moreno ; Timothy G. GearySource :
- PLoS Neglected Tropical Diseases [ 1935-2727 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Brugia malayi (), Brugia malayi (croissance et développement), Brugia malayi (génétique), Brugia malayi (métabolisme), Espace extracellulaire (), Espace extracellulaire (génétique), Espace extracellulaire (métabolisme), Femelle, Filariose lymphatique (parasitologie), Gerbillinae, Mâle, Protéines d'helminthes (), Protéines d'helminthes (génétique), Protéines d'helminthes (métabolisme), Protéomique, Spécificité d'espèce, Étapes du cycle de vie.
- MESH :
- croissance et développement : Brugia malayi.
- génétique : Brugia malayi, Espace extracellulaire, Protéines d'helminthes.
- métabolisme : Brugia malayi, Espace extracellulaire, Protéines d'helminthes.
- parasitologie : Filariose lymphatique.
- Animaux, Brugia malayi, Espace extracellulaire, Femelle, Gerbillinae, Mâle, Protéines d'helminthes, Protéomique, Spécificité d'espèce, Étapes du cycle de vie.
English descriptors
- KwdEn :
- Animals, Brugia malayi (chemistry), Brugia malayi (genetics), Brugia malayi (growth & development), Brugia malayi (metabolism), Elephantiasis, Filarial (parasitology), Extracellular Space (chemistry), Extracellular Space (genetics), Extracellular Space (metabolism), Female, Gerbillinae, Helminth Proteins (chemistry), Helminth Proteins (genetics), Helminth Proteins (metabolism), Life Cycle Stages, Male, Proteomics, Species Specificity.
- MESH :
- chemical , chemistry : Helminth Proteins.
- chemistry : Brugia malayi, Extracellular Space.
- genetics : Brugia malayi, Extracellular Space, Helminth Proteins.
- growth & development : Brugia malayi.
- metabolism : Brugia malayi, Extracellular Space, Helminth Proteins.
- parasitology : Elephantiasis, Filarial.
- Animals, Female, Gerbillinae, Life Cycle Stages, Male, Proteomics, Species Specificity.
Abstract
While we lack a complete understanding of the molecular mechanisms by which parasites establish and achieve protection from host immune responses, it is accepted that many of these processes are mediated by products, primarily proteins, released from the parasite. Parasitic nematodes occur in different life stages and anatomical compartments within the host. Little is known about the composition and variability of products released at different developmental stages and their contribution to parasite survival and progression of the infection.
To gain a deeper understanding on these aspects, we collected and analyzed through 1D-SDS PAGE and LC-MS/MS the Excretory-Secretory Products (ESP) of adult female, adult male and microfilariae of the filarial nematode
A comparative analysis of the proteins released
Url:
DOI: 10.1371/journal.pntd.0000326
PubMed: 18958170
PubMed Central: 2569413
Affiliations:
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Le document en format XML
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Geary</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS Neglected Tropical Diseases</title><idno type="ISSN">1935-2727</idno><idno type="eISSN">1935-2735</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Brugia malayi (chemistry)</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (growth & development)</term><term>Brugia malayi (metabolism)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Extracellular Space (chemistry)</term><term>Extracellular Space (genetics)</term><term>Extracellular Space (metabolism)</term><term>Female</term><term>Gerbillinae</term><term>Helminth Proteins (chemistry)</term><term>Helminth Proteins (genetics)</term><term>Helminth Proteins (metabolism)</term><term>Life Cycle Stages</term><term>Male</term><term>Proteomics</term><term>Species Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Brugia malayi ()</term><term>Brugia malayi (croissance et développement)</term><term>Brugia malayi (génétique)</term><term>Brugia malayi (métabolisme)</term><term>Espace extracellulaire ()</term><term>Espace extracellulaire (génétique)</term><term>Espace extracellulaire (métabolisme)</term><term>Femelle</term><term>Filariose lymphatique (parasitologie)</term><term>Gerbillinae</term><term>Mâle</term><term>Protéines d'helminthes ()</term><term>Protéines d'helminthes (génétique)</term><term>Protéines d'helminthes (métabolisme)</term><term>Protéomique</term><term>Spécificité d'espèce</term><term>Étapes du cycle de vie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Helminth Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Brugia malayi</term><term>Extracellular Space</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term><term>Extracellular Space</term><term>Helminth Proteins</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Brugia malayi</term><term>Espace extracellulaire</term><term>Protéines d'helminthes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Brugia malayi</term><term>Extracellular Space</term><term>Helminth Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Brugia malayi</term><term>Espace extracellulaire</term><term>Protéines d'helminthes</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Gerbillinae</term><term>Life Cycle Stages</term><term>Male</term><term>Proteomics</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Brugia malayi</term><term>Espace extracellulaire</term><term>Femelle</term><term>Gerbillinae</term><term>Mâle</term><term>Protéines d'helminthes</term><term>Protéomique</term><term>Spécificité d'espèce</term><term>Étapes du cycle de vie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Introduction</title><p>While we lack a complete understanding of the molecular mechanisms by which parasites establish and achieve protection from host immune responses, it is accepted that many of these processes are mediated by products, primarily proteins, released from the parasite. Parasitic nematodes occur in different life stages and anatomical compartments within the host. Little is known about the composition and variability of products released at different developmental stages and their contribution to parasite survival and progression of the infection.</p></sec><sec><title>Methodology/Principal Findings</title><p>To gain a deeper understanding on these aspects, we collected and analyzed through 1D-SDS PAGE and LC-MS/MS the Excretory-Secretory Products (ESP) of adult female, adult male and microfilariae of the filarial nematode <italic>Brugia malayi</italic>, one of the etiological agents of human lymphatic filariasis. This proteomic analysis led to the identification of 228 proteins. The list includes 76 proteins with unknown function as well as also proteins with potential immunoregulatory properties, such as protease inhibitors, cytokine homologues and carbohydrate-binding proteins. Larval and adult ESP differed in composition. Only 32 proteins were shared between all three stages/genders. Consistent with this observation, different gene ontology profiles were associated with the different ESP.</p></sec><sec><title>Conclusions/Significance</title><p>A comparative analysis of the proteins released <italic>in vitro</italic> by different forms of a parasitic nematode dwelling in the same host is presented. The catalog of secreted proteins reflects different stage- and gender-specific related processes and different strategies of immune evasion, providing valuable insights on the contribution of each form of the parasite for establishing the host–parasite interaction.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Geary, Timothy G" sort="Geary, Timothy G" uniqKey="Geary T" first="Timothy G." last="Geary">Timothy G. 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